Miller R A, Ruddle F H
Cell. 1976 Sep;9(1):45-55. doi: 10.1016/0092-8674(76)90051-9.
We have produced a series of somatic cell hybrids by fusing pluripotent PCC4aza1 embryonal carcinoma ("teretocarcinoma") cells with thymocytes from young adult mice. When these hybrids form tumors in nu/nu or syngeneic mice, all the tumors contain a range of differentiated tissues, as well as embryonal carcinoma-like tissues. Some of the tumors produce alpha-fetoprotein. These results show that pluripotency in embryonal carcinoma cells need not to be abolished by the introduction of a complete diploid genome from a differentiated cell.
我们通过将多能性的PCC4aza1胚胎癌细胞（“畸胎癌”细胞）与成年幼鼠的胸腺细胞融合，制备了一系列体细胞杂种。当这些杂种在裸鼠或同基因小鼠中形成肿瘤时，所有肿瘤都包含一系列分化组织以及胚胎癌样组织。一些肿瘤会产生甲胎蛋白。这些结果表明，引入来自分化细胞的完整二倍体基因组不一定会消除胚胎癌细胞中的多能性。
