Duhm J, Göbel B O
Hypertension. 1982 Jul-Aug;4(4):468-76. doi: 10.1161/01.hyp.4.4.468.
A reliable and simple uptake test is proposed to assess the activity of the sodium lithium (Na+-Li+) countertransport and the sodium-potassium (Na+-K+) cotransport system of human erythrocytes. The test consists in measurement of phloretin-sensitive Li+ uptake from magnesium (Mg2+) media containing 2 mM Li+ and of furosemide-sensitive rubidium (Rb+) uptake from Na+ media containing 5 mM Rb+. Both Li+ and Rb+ are determined by atomic absorption spectrophotometry. The particular experimental conditions of the uptake assay are analyzed with respect to parameters that potentially affect the results, such as pH, cell volume, internal Na+, external Na+ and Rb+, presence of other ions, and concentration and protein-binding of furosemide, respectively. It is found that the activity of the two transport systems varies more than threefold among normotensive individuals, the activities in erythrocytes of each individual donor remaining stable within +/- 10% over more than two red cell life spans. The Li+ and Rb+ uptake rates are highly correlated to Li+ and K+ or Na+ release rates as determined by the methods of Canessa et al. and Garay et al. The test can thus be applied for routine screening in the search of "abnormalities" of red cell cation transport in hypertensive patients.
本文提出了一种可靠且简单的摄取试验，用于评估人红细胞钠锂（Na⁺-Li⁺）逆向转运和钠钾（Na⁺-K⁺）协同转运系统的活性。该试验包括测量从含2 mM Li⁺的镁（Mg²⁺）培养基中根皮素敏感的Li⁺摄取，以及从含5 mM Rb⁺的Na⁺培养基中呋塞米敏感的铷（Rb⁺）摄取。Li⁺和Rb⁺均通过原子吸收分光光度法测定。分别针对可能影响结果的参数，如pH、细胞体积、细胞内Na⁺、细胞外Na⁺和Rb⁺、其他离子的存在以及呋塞米的浓度和蛋白结合，分析了摄取试验的特定实验条件。研究发现，在血压正常的个体中，这两种转运系统的活性变化超过三倍，每个个体供体红细胞中的活性在超过两个红细胞寿命期内保持在±10%的稳定范围内。Li⁺和Rb⁺摄取率与Canessa等人和Garay等人方法测定的Li⁺和K⁺或Na⁺释放率高度相关。因此，该试验可用于常规筛查，以寻找高血压患者红细胞阳离子转运的“异常”情况。
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