Effmann E L
Invest Radiol. 1982 Nov-Dec;17(6):529-38.
The developing aortic arches and pulmonary vasculature of 200 mouse embryos (gestational age 11.5-18 days) were directly imaged by stereomicroradiography after perfusion fixation, endothelial staining, and critical point drying. At 11.5-days gestational age, symmetric third, fourth, and sixth aortic arches and proximal portions of the right and left pulmonary arteries were noted. At 12 days, rudimentary pulmonary arteries extended dorsocaudally from the middle portion of the left sixth arch, and there was loss of the dorsal component of the right sixth arch. Semilunar valve anlagen were noted at 12 days, and cusps formed during days 13 and 14. With continued growth, the lungs and pulmonary arteries enlarged and came to occupy a more cephalad position relative to the heart. Stereomicroradiographic techniques permit direct imaging of developing vascular systems in the mouse embryo. Information gained from these whole specimen studies serve to complement serial section and reconstruction studies and offer the potential for evaluating normal and abnormal vascular development.
在对200个小鼠胚胎（妊娠年龄11.5 - 18天）进行灌注固定、内皮染色和临界点干燥后，通过立体显微放射摄影对发育中的主动脉弓和肺血管系统进行直接成像。在妊娠11.5天时，可见对称的第三、第四和第六主动脉弓以及左右肺动脉的近端部分。在12天时，原始肺动脉从左第六弓中部向背尾侧延伸，右第六弓的背侧部分消失。在12天时可见半月瓣原基，在第13天和第14天形成瓣叶。随着持续生长，肺和肺动脉增大，并相对于心脏占据更头侧的位置。立体显微放射摄影技术允许对小鼠胚胎中发育的血管系统进行直接成像。从这些全标本研究中获得的信息有助于补充连续切片和重建研究，并为评估正常和异常血管发育提供了潜力。
