Smith B R, Johnson G A, Groman E V, Linney E
Department of Radiology, Duke University Medical Center, Durham, NC 27710.
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3530-3. doi: 10.1073/pnas.91.9.3530.
The increased use of the mouse as a model for various aspects of mammalian biology has caused a renewed interest in developing strategies for examining and comparing normal and abnormal mouse embryonic development and anatomy. In this study, we have explored the use of magnetic resonance microscopy as a tool for these purposes. Techniques for the fixation, embedding, perfusion, and image acquisition of mouse embryos are described. The perfusion of bovine serum albumin-diethylenetriamine pentaacetic anhydride-gadolinium as a contrast agent enhances images of the developing embryonic vasculature during critical stages of organogenesis and allows for comparisons when embryos have been treated with teratogens such as retinoic acid. The acquired three-dimensional data sets are available for archiving, distributing, and postacquisition manipulations such as computer segmentation of anatomical structures.
小鼠作为哺乳动物生物学各个方面的模型使用得越来越多，这引发了人们对开发用于检查和比较正常与异常小鼠胚胎发育及解剖结构的策略的新兴趣。在本研究中，我们探索了将磁共振显微镜用作实现这些目的的工具。描述了小鼠胚胎的固定、包埋、灌注和图像采集技术。灌注牛血清白蛋白 - 二乙烯三胺五乙酸酐 - 钆作为造影剂可增强器官发生关键阶段发育中的胚胎血管系统图像，并能在胚胎用视黄酸等致畸剂处理后进行比较。所获取的三维数据集可用于存档、分发以及采集后操作，如解剖结构的计算机分割。
