采用等度高效液相色谱法测定治疗浓度下的血浆美法仑。

Measurement of plasma melphalan at therapeutic concentrations using isocratic high-performance liquid chromatography.

作者信息

Bosanquet A G, Gilby E D

出版信息

J Chromatogr. 1982 Nov 12;232(2):345-54. doi: 10.1016/s0378-4347(00)84174-2.

Abstract

A sensitive isocratic high-performance liquid chromatographic (HPLC) method for the measurement of melphalan in plasma is presented. It requires an extraction step using columns of XAD-2 resin before injecting the clarified methanol eluate directly into the HPLC system. The HPLC system uses an isocratic mobile phase containing an ion-pair reagent, and a sensitive fixed-wavelength (254 nm) monitor with a noise specification of less than 2 . 10(-5) absorbance units peak to peak. The concentration of melphalan was followed in a patient with multiple myeloma on day 1 and day 4 of a four-day course of the drug. Little difference was detected between the two curves with terminal half-lives of 71 and 68 min respectively and areas under the curve of 1.08 and 1.15 min . microgram/ml . (mg dose)-1.

摘要

本文介绍了一种灵敏的等度高效液相色谱（HPLC）法，用于测定血浆中的美法仑。在将澄清的甲醇洗脱液直接注入HPLC系统之前，需要使用XAD - 2树脂柱进行萃取步骤。HPLC系统使用含有离子对试剂的等度流动相，以及噪声规格小于2×10⁻⁵吸光度单位峰峰值的灵敏固定波长（254 nm）监测器。在一名患有多发性骨髓瘤的患者接受为期四天的药物疗程的第1天和第4天，对美法仑的浓度进行了跟踪。两条曲线之间几乎没有差异，终末半衰期分别为71分钟和68分钟，曲线下面积分别为1.08和1.15分钟·微克/毫升·（毫克剂量）⁻¹。