常规尿液处理的原代培养基
Primary culture media for routine urine processing.
作者信息
Fung J C, Lucia B, Clark E, Berman M, Goldstein J, D'Amato R F
出版信息
J Clin Microbiol. 1982 Oct;16(4):632-6. doi: 10.1128/jcm.16.4.632-636.1982.
It has been recommended that routine microbiological processing of urine specimens include quantitative plating onto blood agar medium along with a selective and differential agar such as MacConkey agar for gram-negative organisms. Few data have been published to justify this combination. To evaluate the validity of this recommendation 2,553 midstream, clean-voided urine samples were quantitatively plated onto blood agar, MacConkey agar, and colistin-nalidixic acid agar, which is a selective medium for gram-positive organisms. The amounts of growth on each of the three media were compared. Results indicated that the best medium combination was colistin-nalidixic acid agar and MacConkey agar. The use of colistin-nalidixic acid agar instead of blood agar increased the detection of significant growth of enterococci, lactobacilli, and Torulopsis glabrata.
有人建议，尿液标本的常规微生物学处理应包括将尿液定量接种到血琼脂培养基上，同时接种一种选择性和鉴别性琼脂，如用于革兰氏阴性菌的麦康凯琼脂。但很少有数据能证明这种组合的合理性。为了评估这一建议的有效性，将2553份中段清洁尿样本定量接种到血琼脂、麦康凯琼脂和多粘菌素-萘啶酸琼脂上，后者是一种用于革兰氏阳性菌的选择性培养基。比较了三种培养基上的生长量。结果表明，最佳培养基组合是多粘菌素-萘啶酸琼脂和麦康凯琼脂。使用多粘菌素-萘啶酸琼脂代替血琼脂可提高肠球菌、乳酸杆菌和光滑球拟酵母菌显著生长的检出率。
Zotero 插件