一种用于检测豆科植物中L-刀豆氨酸的基于精氨酸酶的特定检测方法。
A specific arginase-based assay for L-canavanine in leguminous plants.
作者信息
Korpela T K, Lorenz H, Laakso S
出版信息
J Biochem Biophys Methods. 1982 Dec;7(1):67-70. doi: 10.1016/0165-022x(82)90037-9.
PMID:7153457
Abstract
A convenient method for the analysis of free L-canavanine in leguminous plants is described. Canavanine was specifically hydrolyzed to canaline and urea by the enzyme arginase (EC 3.5.3.1). The resulting amino-oxy functions of canaline were measured based on their ability to bleach the yellow colour of pyridoxal 5'-phosphate. Canavanine in the seeds of Canavalia ensiformis was determined with this method.
摘要
本文描述了一种分析豆科植物中游离L-刀豆氨酸的简便方法。刀豆氨酸通过精氨酸酶（EC 3.5.3.1）特异性水解为刀豆碱和尿素。基于刀豆碱的氨基氧基官能团使5'-磷酸吡哆醛的黄色褪色的能力来测定其含量。用该方法测定了刀豆种子中的刀豆氨酸。
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