Irie T, Otagiri M, Sunada M, Uekama K, Ohtani Y, Yamada Y, Sugiyama Y
J Pharmacobiodyn. 1982 Sep;5(9):741-4. doi: 10.1248/bpb1978.5.741.
Cyclodextrins (CyDs) at higher concentrations were found to cause hemolysis of human erythrocytes in the order of beta- greater than alpha- greater than gamma-CyD in isotonic solution. Biphasic effects of CyDs were observed for the osmotic and heat-induced hemolysis; i.e. the protection at relatively low CyD concentrations and stimulation at higher CyD concentrations. From the scanning electron microscopic observations, CyDs induced shape changes of membrane internalization type on erythrocytes. CyDs caused the release of cholesterol from erythrocyte membrane in the order of beta- greater than gamma- greater than alpha-CyD. These results clearly indicate that CyD-induced hemolysis is probably a secondary event resulting from the membrane disruption which elicited the removal of membrane components from erythrocytes.
研究发现，在等渗溶液中，浓度较高的环糊精（CyDs）会导致人红细胞溶血，其溶血顺序为β-环糊精＞α-环糊精＞γ-环糊精。对于渗透压和热诱导的溶血，观察到环糊精具有双相作用；即在相对较低的环糊精浓度下起到保护作用，而在较高浓度下则产生刺激作用。通过扫描电子显微镜观察发现，环糊精会诱导红细胞发生膜内化类型的形状变化。环糊精导致红细胞膜中胆固醇的释放顺序为β-环糊精＞γ-环糊精＞α-环糊精。这些结果清楚地表明，环糊精诱导的溶血可能是由于膜破坏引发红细胞膜成分去除所导致的继发事件。
