Aktanova Alina, Abramova Tatjana, Pashkina Ekaterina, Boeva Olga, Grishina Lyubov, Kovalenko Ekaterina, Kozlov Vladimir
Research Institute of Fundamental and Clinical Immunology, 6300099 Novosibirsk, Russia.
Department of Medicine, Novosibirsk State University, 630090 Novosibirsk, Russia.
Nanomaterials (Basel). 2021 May 21;11(6):1356. doi: 10.3390/nano11061356.
Currently, cucurbiturils are being actively researched all over the world. Research is focused on the ways of improving the solubility and selectivity of cucurbiturils, increasing the stability of the complexes with other particles in various media and enhancing their ability to bind and release various substances. The most significant area of our research is the assessment of safety, studying the biological properties and synergistic effects of cucurbiturils during complexation with drugs. In this article, the hemocompatibility of erythrocytes and leukocytes with cucurbiturils was investigated. We demonstrated that cucurbiturils have no cytotoxic effect, even at high concentrations (1 mM) and do not affect the viability of PBMCs. However, cucurbiturils can increase the level of the early apoptosis of lymphocytes and cucurbit[7]uril enhances hemolysis in biologically relevant media. Despite this, cucurbiturils are fairly safe organic molecules in concentrations up to 0.3 mM. Thus, we believe that it will become possible to use polymer nanostructures as drug delivery systems in clinical practice, since cucurbiturils can be modified to improve pharmacological properties.
目前，全世界都在积极研究葫芦脲。研究重点在于改善葫芦脲的溶解性和选择性、提高其在各种介质中与其他粒子形成的配合物的稳定性以及增强其结合和释放各种物质的能力。我们研究中最重要的领域是评估安全性，研究葫芦脲在与药物络合过程中的生物学特性和协同效应。在本文中，我们研究了红细胞和白细胞与葫芦脲的血液相容性。我们证明，即使在高浓度（1 mM）下，葫芦脲也没有细胞毒性作用，并且不会影响外周血单核细胞的活力。然而，葫芦脲会增加淋巴细胞早期凋亡水平，葫芦[7]脲会增强生物相关介质中的溶血作用。尽管如此，浓度高达0.3 mM的葫芦脲是相当安全的有机分子。因此，我们相信在临床实践中使用聚合物纳米结构作为药物递送系统将成为可能，因为葫芦脲可以进行修饰以改善药理性质。
