Inada K, Baba H, Okamura R
Jpn J Ophthalmol. 1982;26(3):314-25.
Tears were collected from 10 normal subjects by two methods, 1) micropipetting after stimulation with an onion slice, and 2) Schirmer 1 test using a filter paper strip. The tears in the 5 x 5 mm part of the filter paper placed within the conjunctival sac were extracted with a 0.9% NaCl solution. The tear samples so obtained were analyzed by crossed immunoelectrophoresis. Nineteen proteins were identified by the use of monospecific antisera. Ten tear-specific proteins including the specific tear prealbumin of Bonavida et al., lactoferrin and lysozyme were identified by the intermediate gel method. Some differences were found in the pattern of the tear-specific proteins between the tears collected by the two methods, but the pattern was basically similar. Some individual differences were also found. The levels of serum proteins including albumin and IgG varied considerably, but it was thought to be due to dilution by reflex lacrimation during tear sampling. On the other hand, the pattern of the tear-specific proteins was fairly constant. The filter paper method allowed tear protein analysis, even in patients with 0 mm wetting by the Schirmer 1 test. A study of the length-volume relationship of the filter paper wetting showed that the tears contained in the 5 x 5 mm portion of the filter paper were about 1.8 microliters. The filter paper method was thought to offer a reliable clinical tool for the study of the tear proteins in various pathological conditions of the lacrimal secretion.
通过两种方法从10名正常受试者中收集眼泪：1）用洋葱片刺激后用微量移液器收集；2）使用滤纸条进行Schirmer 1试验。将置于结膜囊内的滤纸5×5mm部分中的眼泪用0.9%氯化钠溶液提取。如此获得的眼泪样本通过交叉免疫电泳进行分析。使用单特异性抗血清鉴定出19种蛋白质。通过中间凝胶法鉴定出10种泪液特异性蛋白质，包括Bonavida等人的特异性泪液前白蛋白、乳铁蛋白和溶菌酶。两种方法收集的眼泪之间，在泪液特异性蛋白质模式上发现了一些差异，但模式基本相似。还发现了一些个体差异。包括白蛋白和IgG在内的血清蛋白水平差异很大，但认为这是由于泪液采样期间反射性流泪导致的稀释。另一方面，泪液特异性蛋白质的模式相当恒定。即使是Schirmer 1试验湿润长度为0mm的患者，滤纸法也能进行泪液蛋白质分析。对滤纸湿润的长度-体积关系的研究表明，滤纸5×5mm部分所含的眼泪约为1.8微升。滤纸法被认为是研究泪液分泌各种病理状况下泪液蛋白质的可靠临床工具。
