Boukes R J, Boonstra A, Breebaart A C, Reits D, Glasius E, Luyendyk L, Kijlstra A
Department of Ophthalmology, Hospital of the Free University, Amsterdam, The Netherlands.
Doc Ophthalmol. 1987 Sep-Oct;67(1-2):105-13. doi: 10.1007/BF00142704.
Using high performance liquid chromatography (HPLC) the tear protein profiles were measured in controls, patients with Sjögren's disease, questionable dry eye (idiopathic dry eye), idiopathic chronic conjunctivitis and the corneal melting syndrome. Qualitative comparison of the protein profiles of patients with Sjögren's disease, corneal melting and IgA deficiency shows a marked difference in the heights of various peaks as compared to the profiles of the control group. The total protein content of tears in controls and in patients with idiopathic chronic conjunctivitis is age dependent and appears to increase until the age of 40 and to decrease afterwards. The peaks containing IgA, lactoferrin and lysozyme were measured in various eye diseases. In idiopathic chronic conjunctivitis and in the corneal melting syndrome no differences were seen in comparison with controls. In patients with idiopathic dry eye and Sjögren's disease a marked decrease in the three proteins was seen. The study presented here indicates that the HPLC analysis of tears is a promising technique which may increase our knowledge of this ocular fluid.
采用高效液相色谱法（HPLC）对对照组、干燥综合征患者、可疑干眼（特发性干眼）、特发性慢性结膜炎和角膜溶解综合征患者的泪液蛋白质谱进行了测定。干燥综合征、角膜溶解和IgA缺乏患者的蛋白质谱与对照组相比，各峰高度存在显著差异。对照组和特发性慢性结膜炎患者泪液中的总蛋白含量与年龄有关，在40岁之前似乎会增加，之后则会下降。在各种眼部疾病中对含IgA、乳铁蛋白和溶菌酶的峰进行了测定。与对照组相比，特发性慢性结膜炎和角膜溶解综合征患者未见差异。在特发性干眼和干燥综合征患者中，这三种蛋白质明显减少。本文介绍的研究表明，泪液的HPLC分析是一种很有前景的技术，可能会增加我们对这种眼液的了解。
