Greenfield S A, Shaw S G
Neuroscience. 1982;7(11):2883-93. doi: 10.1016/0306-4522(82)90111-7.
Push-pull cannulae were implanted acutely in both substantiae nigrae and both caudate nuclei of the rabbit under urethane anesthesia. Direct infusion of d-amphetamine (10(-6) M) to one substantia nigra evoked a release of acetylcholinesterase and aminopeptidase not only locally, but also from the contralateral caudate nucleus and substantia nigra. The spontaneous release of acetylcholinesterase was reduced in the ipsilateral caudate nucleus. Gel electrophoresis showed that only one molecular form of each enzyme was released. Since the electrophoretic mobilities of these two enzymic activities were different, the released acetylcholinesterase and aminopeptidase belong to separate molecular species. As amphetamine is known to modify dopamine metabolism the evoked release of these two enzymes is probably related to the dopamine-containing nigrostriatal system.
在氨基甲酸乙酯麻醉下，将推挽式套管急性植入兔的双侧黑质和双侧尾状核。向一侧黑质直接注入d-苯丙胺（10^(-6) M）不仅引起局部乙酰胆碱酯酶和氨肽酶的释放，还引起对侧尾状核和黑质的释放。同侧尾状核中乙酰胆碱酯酶的自发释放减少。凝胶电泳显示每种酶仅释放一种分子形式。由于这两种酶活性的电泳迁移率不同，释放的乙酰胆碱酯酶和氨肽酶属于不同的分子种类。由于已知苯丙胺会改变多巴胺代谢，这两种酶的诱发释放可能与含多巴胺的黑质纹状体系统有关。
