Lachâtre G F, Nicot G S, Merle L J, Valette J P
Ther Drug Monit. 1982;4(4):359-64. doi: 10.1097/00007691-198212000-00005.
The authors describe a method for the plasma determination of mianserin by gas--liquid chromatography (GLC), using cyproheptadine as an internal standard. After extraction from 2 ml of plasma by a hexane:isoamyl alcohol (99:1, vol/vol) mixture and its purification by reextraction, the mianserin is determined by GLC, using a glass column packed with 3% OV1 on Gas-Chrom Q, and a nitrogen-sensitive detector. Since the between-day coefficient of variation (n = 15) is less than 8%, this technique appears to be sufficiently reproducible for routine determination in therapeutic concentrations (detection limit with accuracy = 2.5 microgram/L). As an example, the authors describe the time variations of the plasma concentration of mianserin after a first-dose ingestion of 30 mg. Preliminary studies of kinetic parameters are determined in 11 adult patients following a single oral dose (30 mg of mianserin).
作者描述了一种用气相-液相色谱法（GLC）测定血浆中米安色林的方法，使用赛庚啶作为内标。用己烷：异戊醇（99:1，体积/体积）混合物从2毫升血浆中萃取，经再萃取纯化后，用GLC测定米安色林，使用填充有3% OV1的Gas-Chrom Q玻璃柱和氮敏感检测器。由于日间变异系数（n = 15）小于8%，该技术对于治疗浓度的常规测定似乎具有足够的重现性（检测限，准确度为2.5微克/升）。作为一个例子，作者描述了首次服用30毫克米安色林后血浆中米安色林浓度随时间的变化。在11名成年患者单次口服剂量（30毫克米安色林）后测定了动力学参数的初步研究。
