Kang K Y, Salvaggio J
Tohoku J Exp Med. 1978 Dec;126(4):317-24. doi: 10.1620/tjem.126.317.
Alveolar macrophages from the rabbit were exposed in the culture medium to zirconium and aluminum salts. The specific activities of the lysosomal hydrolases, that is acid phosphatase, beta-N-acetylglucosaminidase and beta-glucuronidase, were measured in the medium, whole cell homogenate, mitochondrial fraction, and in the supernatant fraction. A highly significant increase of these hydrolases was observed in the mitochondrial fraction from cells exposed to zirconium and aluminum salts as compared with those from control cell cultures. However, release of these enzymes into the medium was not much. The phenomenon of macrophage phagocytosis was observed morphologically in the cell cultures exposed in vitro to these metal compounds.
将兔肺泡巨噬细胞置于含有锆盐和铝盐的培养基中。在培养基、全细胞匀浆、线粒体组分以及上清液组分中测定溶酶体水解酶（即酸性磷酸酶、β-N-乙酰氨基葡萄糖苷酶和β-葡萄糖醛酸酶）的比活性。与对照细胞培养物相比，暴露于锆盐和铝盐的细胞线粒体组分中这些水解酶的活性显著增加。然而，这些酶释放到培养基中的量并不多。在体外暴露于这些金属化合物的细胞培养物中，从形态学上观察到了巨噬细胞的吞噬现象。
