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[测定不同植物源性食品中黄曲霉毒素含量的改进方法]

[Improved method of determining the aflatoxin content in different food products of plant origin].

作者信息

Eller K I, Maksimenko L V, Tutel'ian V A

出版信息

Vopr Pitan. 1982 Nov-Dec(6):62-6.

PMID:7157744
Abstract

The authors offer an improved method for detection, identification and quantitation of aflatoxins, fit for series analyses. The method includes extract purification by means of two-dimensional thin-layer chromatography and quantitative determination of aflatoxin from their specific fluorescence. Combination of the techniques included in the method enables one to separate aflatoxin from interfering substances in extracts of any products of plant origin and excludes the attainment of pseudopositive results. The method was made use of for studying contamination of 145 samples of foods. Aflatoxin B1 was found in some of the samples. The maximal content of the aflatoxin was detected in corn and peanut. Apart from aflatoxin B1, the corn samples were demonstrated to contain aflatoxins B2, G1, and G2.

摘要

作者提供了一种改进的黄曲霉毒素检测、鉴定和定量方法,适用于系列分析。该方法包括通过二维薄层色谱法进行提取物纯化,并根据其特定荧光对黄曲霉毒素进行定量测定。该方法中所包含技术的结合,能够从任何植物源产品提取物中的干扰物质中分离出黄曲霉毒素,并排除假阳性结果的出现。该方法被用于研究145份食品样本的污染情况。在部分样本中发现了黄曲霉毒素B1。在玉米和花生中检测到了黄曲霉毒素的最大含量。除黄曲霉毒素B1外,玉米样本还被证明含有黄曲霉毒素B2、G1和G2。

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