Yanai J, Woolf M, Feigenbaum J J
Acta Anat (Basel). 1982;114(4):347-54. doi: 10.1159/000145607.
Pregnant Sabra mice received 3 g phenobarbital per kilogram milled food from gestational days (GD) 9 to 18 as their only food source; control females received milled food. All the females were divided into three groups and injected intraperitoneally with a single dose of 3H-thymidine on GD 13, 15 or 17. At 50 days of age of the offspring were perfused with 10% neutral formalin and their brains removed and prepared for autoradiography. Matching sagittal sections of the offspring were selected for the study of the medial preoptic area (MPOA) of the hypothalamus. The total number of cells per section was not significantly different between males and females, however, there was a greater cellular packing density (cell/mm2) in females (p less than 0.05). This sex difference disappeared in treated offspring, possibly due to a 76% decrease on GD 13 in the rate of cell proliferation in females (number of labeled cells but not in males (p less than 0.001); the greatest decrease in the number of labeled cells occurred in the sexually dimorphic areas of the MPOA (p less than 0.001). The area of the sagittal section of the MPOA did not differ significantly between sexes or treatments.
怀孕的Sabra小鼠从妊娠第9天到第18天，每千克碾碎食物中摄入3克苯巴比妥作为唯一食物来源；对照雌性小鼠则食用碾碎食物。所有雌性小鼠分为三组，在妊娠第13、15或17天腹腔注射单剂量的3H-胸腺嘧啶核苷。子代50日龄时，用10%中性福尔马林灌注，取出大脑并准备进行放射自显影。选择子代匹配的矢状切片用于研究下丘脑内侧视前区（MPOA）。每切片细胞总数在雄性和雌性之间无显著差异，然而，雌性的细胞堆积密度（细胞/mm²）更高（p小于0.05）。这种性别差异在经处理的子代中消失，可能是由于雌性在妊娠第13天细胞增殖率下降了76%（标记细胞数量下降，而雄性未下降，p小于0.001）；标记细胞数量下降最多的是MPOA的性二态区（p小于0.001）。MPOA矢状切片面积在性别或处理组之间无显著差异。
