Bergman A, Roselli-Austin L, Yedwab G, Yanai J
Acta Anat (Basel). 1980;108(3):370-3. doi: 10.1159/000145319.
Four groups of pregnant mice were fed milled food containing 3 g/kg phenobarbital, acid form, and water as their only nutritional source during various periods during pregnancy (days 9-18, 9-13, 13-16 or 16-18). Control females received milled food and water. The brains of control and phenobarbital-treated male offspring were removed at age 50 days, fixed, cut and stained with HE. Matching sagittal sections were sections were selected for the study of the cerebellar Purkinje cells and th hippocampal pyramidal cells. Previous findings were confirmed in that prenatal phenobarbital exposure during the last two trimesters of pregnancy (days 9-18) resulted in a long-lasting deficit in the number of the Purkinje (23%) and pyramidal cells 9% (P < 0.01). Phenobarbital administration during only part of this period (days 9-13, 13-16 or 16-18) had the same effect as administration during the entire period. The cell deficit did not correlate with the time of appearance of the neurons, thus, suggesting that phenobarbital may possibly act even on neurons which are already formed.
将四组怀孕小鼠在孕期的不同时间段（第9 - 18天、第9 - 13天、第13 - 16天或第16 - 18天）喂以含有3克/千克苯巴比妥（酸形式）的碾碎食物，并将水作为它们唯一的营养来源。对照组雌性小鼠则给予碾碎食物和水。在50日龄时取出对照组和经苯巴比妥处理的雄性后代的大脑，进行固定、切片并用苏木精-伊红染色。选择匹配的矢状切片用于研究小脑浦肯野细胞和海马锥体细胞。先前的研究结果得到证实，即孕期最后两个阶段（第9 - 18天）产前暴露于苯巴比妥会导致浦肯野细胞数量长期减少23%，锥体细胞数量减少9%（P < 0.01）。仅在该时期的部分时间（第9 - 13天、第13 - 16天或第16 - 18天）给予苯巴比妥与在整个时期给予具有相同效果。细胞数量减少与神经元出现的时间无关，因此表明苯巴比妥甚至可能对已经形成的神经元起作用。
