de Pomerai D I, Gali M A
Adv Exp Med Biol. 1982;158:199-207. doi: 10.1007/978-1-4899-5292-9_21.
Adult sera (horse, chicken and newborn bovine serum) do not support extensive transdifferentiation of lens cells in cultures of 9-day chick embryo neural retina. Conversely, both chick embryo extract and foetal calf serum promote the accumulation of delta-crystallin (a marker for lens cells) in such cultures. Dialysed foetal calf serum does not allow transdifferentiation into lens, whereas the dialysis medium is able to do so in the absence of macromolecular serum components, suggesting one or more active factors of low molecular weight. Choline acetyltransferase activity (cholinergic neuronal marker) is generally maintained for longer under conditions which do not permit extensive transdifferentiation. Glutamine synthetase activity (a glial marker) is inducible by hydrocortisone in dense neuro-retinal cultures, and this hormone also reduces the extent of later lens development.
在9日龄鸡胚神经视网膜培养物中，成年血清（马血清、鸡血清和新生牛血清）不支持晶状体细胞的广泛转分化。相反，鸡胚提取物和胎牛血清都能促进此类培养物中δ-晶状体蛋白（晶状体细胞的标志物）的积累。经透析的胎牛血清不允许转分化为晶状体，而透析培养基在没有大分子血清成分的情况下能够做到这一点，这表明存在一种或多种低分子量的活性因子。胆碱乙酰转移酶活性（胆碱能神经元标志物）在不允许广泛转分化的条件下通常能维持更长时间。谷氨酰胺合成酶活性（一种神经胶质标志物）在致密的神经视网膜培养物中可被氢化可的松诱导，并且这种激素也会降低后期晶状体发育的程度。
