Edén S, Kostyo J L, Schwartz J
Biochim Biophys Acta. 1982 Dec 30;721(4):489-91. doi: 10.1016/0167-4889(82)90106-9.
Several noncovalent complexes of large fragments of human GH, which are less active than native human GH in stimulating glucose metabolism in adipose tissue of hypophysectomized rats, were tested for their ability to compete with 125I-iodinated human GH for specific binding to isolated adipocytes of hypophysectomized rats. The complexes tested were A (residues 1-134 + residues 141-191; S-carbamidomethylated), B (residues 1-134 + residues 135-191; S-carbamidomethylated) and C (residues 1-134 + residues 135-191; S-carboxymethylated). When compared to native human GH, the complexes were less active in competing with 125I-iodinated human GH for specific binding to adipocytes, and their order of potency in the binding assay (A greater than B greater than C) was similar to that of their respective activities in stimulating glucose metabolism in isolated adipose tissue of hypophysectomized rats.
对几种人生长激素（GH）大片段的非共价复合物进行了测试，这些复合物在刺激垂体切除大鼠脂肪组织中的葡萄糖代谢方面比天然人GH活性低，测试它们与125I标记的人GH竞争结合垂体切除大鼠分离脂肪细胞的能力。所测试的复合物为A（残基1 - 134 + 残基141 - 191；S - 氨基甲酰甲基化）、B（残基1 - 134 + 残基135 - 191；S - 氨基甲酰甲基化）和C（残基1 - 134 + 残基135 - 191；S - 羧甲基化）。与天然人GH相比，这些复合物在与125I标记的人GH竞争结合脂肪细胞方面活性较低，并且它们在结合试验中的效力顺序（A大于B大于C）与其在刺激垂体切除大鼠分离脂肪组织中葡萄糖代谢的各自活性顺序相似。
