二聚体和四聚体血影蛋白的溶液散射研究

Solution scattering studies of dimeric and tetrameric spectrin.

作者信息

Reich M H, Kam Z, Eisenberg H, Worcester D, Ungewickell E, Gratzer W B

出版信息

Biophys Chem. 1982 Dec;16(4):307-16. doi: 10.1016/0301-4622(82)87035-x.

Abstract

The structure of spectrin dimers and tetramers in solution has been examined by light, low-angle X-ray and neutron scattering. The results show a good correspondence between the solution dimensions of these molecules and their appearance in the electron microscope after shadowing. The scattering profiles are not compatible with an extended rod-like character, but reflect the presence of a considerable degree of bending. The radii of gyration of the dimer and tetramer were determined to be 170 and 375 A and the cross-section radii of gyration 14 and 12.3 A, respectively. Both are thus long, thin, rather bent molecules, and the tetramer is twice the length of the dimer.

摘要

已通过光散射、低角度X射线散射和中子散射研究了溶液中血影蛋白二聚体和四聚体的结构。结果表明，这些分子在溶液中的尺寸与其在电子显微镜下经投影后的外观具有良好的对应关系。散射曲线与伸展的棒状特征不相符，而是反映出存在相当程度的弯曲。二聚体和四聚体的回转半径分别确定为170 Å和375 Å，截面回转半径分别为14 Å和12.3 Å。因此，二者均为长而细、弯曲程度较大的分子，且四聚体的长度是二聚体的两倍。