Goldberg M A, Lalos A T, Himmelstein B, Bunn H F
Blood Cells. 1982;8(2):237-43.
We have investigated the effect of erythrocyte membrane preparations on the rate of polymerization and the solubility of deoxyhemoglobin S. The kinetics of polymerization were monitored by measuring changes in turbidity of hemoglobin S solutions as a function of time in a temperature-controlled cuvette. The solubility of deoxyhemoglobin S, an equilibrium measurement, was determined by measuring the hemoglobin concentration of the supernatant following centrifugation of the polymerized hemoglobin S solution. The solubility of deoxyhemoglobin S was unaffected by the presence of red cell membrane ghosts, inside-out red cell vesicles, or inside-out vesicles enriched in erythrocyte spectrin and actin (Bands 1, 2, and 5) in the hemoglobin solution. Furthermore, inside-out vesicles had no significant effect on the kinetics of polymerization. The addition of erythrocyte membrane ghosts to the hemoglobin solution, in ratios approximating that of the intact red cell, increased the rate of polymerization up to 8-fold. However, when corrections were made for the excluded volume contributed by the membrane ghosts, the enhancement of the rate of polymerization was reduced to less than 3-fold. These results fail to show significant facilitation of intracellular sickling by nucleation sites on the red cell membrane.
我们研究了红细胞膜制剂对脱氧血红蛋白S聚合速率和溶解度的影响。通过在温度可控的比色皿中测量血红蛋白S溶液的浊度随时间的变化来监测聚合动力学。脱氧血红蛋白S的溶解度是一种平衡测量值，通过测量聚合血红蛋白S溶液离心后上清液中的血红蛋白浓度来确定。血红蛋白溶液中存在红细胞膜空泡、外翻红细胞囊泡或富含红细胞血影蛋白和肌动蛋白（带1、2和5）的外翻囊泡时，脱氧血红蛋白S的溶解度不受影响。此外，外翻囊泡对聚合动力学没有显著影响。以接近完整红细胞的比例向血红蛋白溶液中添加红细胞膜空泡，可使聚合速率提高至8倍。然而，当对膜空泡贡献的排除体积进行校正后，聚合速率的提高降至不到3倍。这些结果未能显示红细胞膜上的成核位点对细胞内镰状化有显著促进作用。
