Schliwa M, Pryzwansky K B, Euteneuer U
Cell. 1982 Dec;31(3 Pt 2):705-17. doi: 10.1016/0092-8674(82)90325-7.
Detergent-extracted whole mount preparations of human neutrophilic polymorphonuclear leukocytes (neutrophils) were used to study centrosome position and microtubule organization during random, chemokinetic and chemotactic locomotion. Chemotaxing neutrophils have a polarized external and internal morphology, with centrosomes between the anterior lamellipod and the nucleus. In randomly locomoting cells centrosome position is highly variable with respect to these two cell organelles. Stimulation of randomly locomoting cells with a chemoattractant (chemokinesis) rapidly (within 1 min) induces pronounced spreading and ruffling and concomitantly initiates, in up to 55% of the cells, transient separation of the centrosome into two solitary centrioles, each surrounded by an aster of microtubules. In addition, about 10% of the cells with separated centrosomes have a third, centriole-free aster of microtubules radiating from an accumulation of dense material. Neutrophil activation also leads to an increase in the number of microtubules and an approximately twofold increase in polymer length. This unusual response of the centrosome supports a correlation between cell movement and microtubule organization.
采用去污剂提取的人嗜中性多形核白细胞（中性粒细胞）整装标本，研究随机运动、化学动力学运动和趋化性运动过程中的中心体位置和微管组织。趋化运动的中性粒细胞具有极化的外部和内部形态，中心体位于前板层伪足和细胞核之间。在随机运动的细胞中，相对于这两个细胞器，中心体位置高度可变。用趋化因子刺激随机运动的细胞（化学激活）会迅速（在1分钟内）诱导明显的铺展和褶皱，并同时在高达55%的细胞中引发中心体短暂分离为两个单独的中心粒，每个中心粒被微管星状体包围。此外，约10%中心体分离的细胞有第三个无中心粒的微管星状体，从致密物质聚集体发出。中性粒细胞激活还会导致微管数量增加，聚合物长度增加约两倍。中心体的这种异常反应支持了细胞运动与微管组织之间的相关性。
