Euteneuer U, Schliwa M
J Cell Biol. 1985 Jul;101(1):96-103. doi: 10.1083/jcb.101.1.96.
Cultured human polymorphonuclear leukocytes exposed to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) spread on the substratum and undergo centrosome splitting. The two centrioles may separate by a distance of several micrometers, each being surrounded by an aster of microtubules. Here we show that the centriole/aster complexes are in constant, rapid motion through the cytoplasm, carrying with them some of the cytoplasmic granules while pushing aside others, or deforming and displacing the nucleus. An analysis of this unique motility phenomenon was undertaken. We show that intact microtubules are required for TPA-induced centrosome splitting and aster motility, but not for cell spreading. More importantly, disruption of the actin network inhibits both centrosome splitting and cell spreading, and even reverses splitting (induces convergence and fusion of asters) in polymorphonuclear leukocytes pretreated with TPA alone. These observations indicate the existence of a dynamic relationship between microtubules and actin networks and provide evidence for a role of actin in determining the position of the centrosome by way of interaction with the microtubules radiating from it.
将人多形核白细胞培养物暴露于肿瘤启动子12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）下，细胞会在基质上铺展并发生中心体分裂。两个中心粒可能分开几微米的距离，每个中心粒都被微管星状体围绕。在这里，我们表明中心粒/星状体复合物在细胞质中持续快速移动，它们会携带一些细胞质颗粒，同时推开其他颗粒，或者使细胞核变形和移位。我们对这种独特的运动现象进行了分析。我们发现，完整的微管是TPA诱导中心体分裂和星状体运动所必需的，但对于细胞铺展并非必需。更重要的是，肌动蛋白网络的破坏会抑制中心体分裂和细胞铺展，甚至在仅用TPA预处理的多形核白细胞中使分裂逆转（诱导星状体的汇聚和融合）。这些观察结果表明微管和肌动蛋白网络之间存在动态关系，并为肌动蛋白通过与从中心体辐射出的微管相互作用来确定中心体位置的作用提供了证据。
