关于脑啡肽与去甲肾上腺素在牛肾上腺髓质颗粒中共存的证据不足。

Evidence against co-storage of enkephalins with noradrenaline in bovine adrenal medullary granules.

作者信息

Lang R E, Taugner G, Gaida W, Ganten D, Kraft K, Unger T, Wunderlich I

出版信息

Eur J Pharmacol. 1982 Dec 17;86(1):117-20. doi: 10.1016/0014-2999(82)90408-3.

Abstract

Bovine adrenal chromaffin granules were purified and separated into adrenaline (A) and noradrenaline (NA) containing granule populations by a combination of differential and isopycnic gradient centrifugation. The distribution of [Leu5]enkephalin as measured by radioimmunoassay followed a pattern identical to adrenaline but not noradrenaline on centrifugation. This suggests that enkephalins are stored in the same type of granule where adrenaline is found or that enkephalins and adrenaline even coexist in one and the same granule. Enkephalins may therefore function as co-transmitters or co-hormones of adrenaline rather than noradrenaline in the adrenal medulla.

摘要

牛肾上腺嗜铬颗粒经差速离心和等密度梯度离心相结合的方法进行纯化，并分离为含肾上腺素（A）和去甲肾上腺素（NA）的颗粒群体。通过放射免疫测定法测得的[亮氨酸5]脑啡肽的分布在离心时呈现出与肾上腺素相同而非去甲肾上腺素的模式。这表明脑啡肽储存在发现肾上腺素的同一类型颗粒中，或者脑啡肽和肾上腺素甚至共存于同一个颗粒中。因此，在肾上腺髓质中，脑啡肽可能作为肾上腺素而非去甲肾上腺素的共递质或共激素发挥作用。