用于tRNA三级结构的化学探针。tRNA与甲基亚硝基脲、乙基亚硝基脲和硫酸二甲酯的比较烷基化反应。

Chemical probes for tRNA tertiary structure. Comparative alkylation of tRNA with methylnitrosourea, ethylnitrosourea and dimethylsulfate.

作者信息

Barciszewski J, Romby P, Ebel J P, Giegé R

出版信息

FEBS Lett. 1982 Dec 27;150(2):459-64. doi: 10.1016/0014-5793(82)80789-8.

DOI:10.1016/0014-5793(82)80789-8

Abstract

The tertiary structure of tRNA in solution can be proved by chemical modification experiments. Three reagents, N-ethyl-N-nitrosourea, N-methyl-N-nitrosourea and dimethylsulfate which are known to alkylate nucleic acids at nucleophilic centers were compared. It is found that N-ethyl-N-nitrosourea and N-methyl-N-nitrosourea mainly react with phosphate residues and dimethylsulfate only with the bases. With dimethylsulfate the extent of alkylation of guanosines is about one order of magnitude higher than that of the phosphates by the nitroso compounds.

摘要

溶液中转运核糖核酸（tRNA）的三级结构可通过化学修饰实验得到证实。比较了三种已知能在亲核中心使核酸烷基化的试剂：N-乙基-N-亚硝基脲、N-甲基-N-亚硝基脲和硫酸二甲酯。结果发现，N-乙基-N-亚硝基脲和N-甲基-N-亚硝基脲主要与磷酸残基反应，而硫酸二甲酯仅与碱基反应。使用硫酸二甲酯时，鸟苷的烷基化程度比亚硝基化合物对磷酸盐的烷基化程度高约一个数量级。

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