Marushige K, Marushige Y
Chem Biol Interact. 1983 Sep 1;46(2):179-88. doi: 10.1016/0009-2797(83)90027-3.
Alkylation of DNA with N-methyl-N-nitrosourea (MeNU) and N-ethyl-N-nitrosourea (EtNU) reduces its ability to support RNA synthesis catalyzed by exogenously added RNA polymerase. It is likely that 7-alkylguanine and alkyl phosphotriester in DNA are mainly responsible for the inhibition of RNA synthesis. The inhibitory effect of alkyl groups varies depending upon divalent metal ions and the type of RNA polymerase used as well as upon the presence of chromosomal proteins on DNA templates. Analyses of RNA products indicate that inhibition occurs primarily at the initiation step.
用N-甲基-N-亚硝基脲（MeNU）和N-乙基-N-亚硝基脲（EtNU）对DNA进行烷基化处理会降低其支持外源添加的RNA聚合酶催化RNA合成的能力。DNA中的7-烷基鸟嘌呤和烷基磷酸三酯可能是抑制RNA合成的主要原因。烷基的抑制作用因二价金属离子、所用RNA聚合酶的类型以及DNA模板上染色体蛋白的存在情况而异。对RNA产物的分析表明，抑制主要发生在起始步骤。
