Nohl H
Gerontology. 1982 Nov-Dec;28(6):354-9. doi: 10.1159/000212556.
Morphological changes in rat heart mitochondria following mobilization of the adenine nucleotide binding sites from the matrix to the cytosolic face of the inner membrane have been documented in this paper by means of electron microscopy. Significant differences in the shape of cristae membranes could be established when rat heart mitochondria from 3- and 30-month-old animals were compared. Changes in structural organization of the inner membrane - an event associated with the function of the adenine nucleotide carrier - were found to be distinctly less marked in mitochondria from the aged group. Kinetic analysis of this process revealed that both the velocity and the extent of conformational membrane changes were reduced as the animal ages. These observations are discussed in relation to the finding, also described in this paper, that aging is linked to a drastic decrease in the activity of adenosine diphosphate/adenosine triphosphate exchange.
本文通过电子显微镜记录了大鼠心脏线粒体中腺嘌呤核苷酸结合位点从线粒体内膜基质面转移至胞质面后发生的形态学变化。比较3月龄和30月龄大鼠心脏线粒体时，可发现嵴膜形状存在显著差异。内膜结构组织的变化——这一与腺嘌呤核苷酸载体功能相关的事件——在老年组大鼠的线粒体中明显不那么显著。对这一过程的动力学分析表明，随着动物年龄增长，膜构象变化的速度和程度均降低。本文还描述了衰老与二磷酸腺苷/三磷酸腺苷交换活性急剧下降有关这一发现，并结合这些观察结果进行了讨论。
