Pisarenko O I, Solomatina E S, Studneva I M
Biokhimiia. 1987 Apr;52(4):543-9.
The effect of acute respiratory hypoxia in rats on mitochondrial respiration, adenine nucleotides and some amino acids of the heart was studied. The decrease in the total (ATP + ADP + AMP) and exchangeable (ATP + ADP) adenine nucleotide pool of the mitochondria was accompanied by a pronounced loss of state 3 respiration with glutamate plus malate and a slight decrease with succinate plus rothenone. The uncoupled respiration of mitochondria with glutamate and malate was decreased in the same degree as in the absence of 2,4-dinitrophenol. State 4 respiration with substrates of both types was unaffected by hypoxia. These data point to a hypoxia-induced impairment of complex I of the respiratory chain. The decrease of tissue and mitochondrial glutamate was accompanied by the elevation of alanine content in the heart and an increase in intramitochondrial aspartate. The ADP-stimulated respiration of mitochondria was correlated with mitochondrial glutamate and ATP as well as with exchangeable adenine nucleotide pools during hypoxia. The experimental results suggest that mitochondrial dysfunction induced by hypoxia may also be attributed to the low level of mitochondrial glutamate.
研究了大鼠急性呼吸性缺氧对心脏线粒体呼吸、腺嘌呤核苷酸和某些氨基酸的影响。线粒体总（ATP + ADP + AMP）和可交换（ATP + ADP）腺嘌呤核苷酸池的减少伴随着谷氨酸加苹果酸时状态3呼吸的显著丧失以及琥珀酸加鱼藤酮时的轻微减少。谷氨酸和苹果酸存在时线粒体的解偶联呼吸与不存在2,4-二硝基苯酚时下降程度相同。两种底物的状态4呼吸不受缺氧影响。这些数据表明呼吸链复合体I存在缺氧诱导的损伤。组织和线粒体谷氨酸的减少伴随着心脏中丙氨酸含量的升高以及线粒体内天冬氨酸的增加。缺氧期间，线粒体的ADP刺激呼吸与线粒体谷氨酸、ATP以及可交换腺嘌呤核苷酸池相关。实验结果表明，缺氧诱导的线粒体功能障碍也可能归因于线粒体谷氨酸水平较低。
