Kalimi M
Gerontology. 1982 Nov-Dec;28(6):371-6. doi: 10.1159/000212558.
The specific [3H]-dexamethasone binding ability of the cytosols of divergent tissues such as kidney, testis and heart were examined in adult (2- to 3-month-old) as well as in senescent (20- to 24-month-old) male adrenalectomized Sprague-Dawley rats. Scatchard analysis showed the presence of a single class of high affinity binding sites in all these tissues from both adult and aged animals. No significant age-related changes in receptor binding site concentration, affinity or steroid specifities were apparent in either kidney, testis or heart. In addition, no age-related changes were observed in in vivo or in vitro nuclear binding of the [3H]-dexamethasone-receptor complexes in all the tissues examined. It was concluded that aging does not alter glucocorticoid receptor concentration, affinity or its ability to bind to nuclei in these diverse tissues examined.
在成年（2至3月龄）以及衰老（20至24月龄）的雄性肾上腺切除的Sprague-Dawley大鼠中，检测了不同组织（如肾脏、睾丸和心脏）细胞质中[3H]-地塞米松的特异性结合能力。Scatchard分析表明，成年和老年动物的所有这些组织中均存在单一类别的高亲和力结合位点。在肾脏、睾丸或心脏中，受体结合位点浓度、亲和力或类固醇特异性均未出现明显的与年龄相关的变化。此外，在所检测的所有组织中，[3H]-地塞米松-受体复合物的体内或体外核结合均未观察到与年龄相关的变化。结论是，衰老不会改变所检测的这些不同组织中糖皮质激素受体的浓度、亲和力或其与细胞核结合的能力。
