Garberi J C, Fontana J D, Blaquier J A
Int J Androl. 1982 Dec;5(6):619-26. doi: 10.1111/j.1365-2605.1982.tb00296.x.
Previous results demonstrated that androgen-dependent rat specific epididymal proteins (SEP) were bound to spermatozoa during their maturation in the epididymis. This paper describes the purification of glycoprotein DE, which constitutes 40% of SEP, and the identification and semiquantitative determination of the sugars forming its oligosaccharide chain. Affinity chromatography on Sepharose-Concanavalin A produced a sample of D-E 95% pure in which 10.5 g of sugar were present per 100 g protein. The percentual composition of the oligosaccharide was D-mannose 19%; D-galactose 3%; N-acetyl-D-glucosamine 33%; N-acetyl-neuraminic acid 31% and D-glucose 13%.
先前的研究结果表明，雄激素依赖性大鼠特异性附睾蛋白（SEP）在精子于附睾中成熟的过程中与精子结合。本文描述了糖蛋白DE的纯化过程，该糖蛋白占SEP的40%，并对构成其寡糖链的糖类进行了鉴定和半定量测定。用琼脂糖-伴刀豆球蛋白A进行亲和层析得到了纯度为95%的D-E样品，其中每100克蛋白质中含有10.5克糖。寡糖的百分比组成为：D-甘露糖19%；D-半乳糖3%；N-乙酰-D-葡萄糖胺33%；N-乙酰神经氨酸31%；D-葡萄糖13%。
