Bogema S C, Narasimhachari N, Mumtaz M, Goldin S, Friedel R O
J Chromatogr. 1982 Dec 10;233:257-67. doi: 10.1016/s0378-4347(00)81752-1.
A high-performance liquid chromatographic procedure is described for the separation of cis and trans isomers of thiothixene, a thioxanthene derivative used as an antipsychotic agent. A radial compression module (RCM-100) was used with both silica and cyanopropyl cartridges. A fixed-wavelength UV detector (254 nm) was used in these studies for quantitation. Mesoridazine is used as an internal standard because of its separation characteristics and reproducible quantitation. C18 Sep-Pak cartridges are used for biological sample clean-up. Plasma samples from patients treated with thiothixene (Navane) were assayed for cis and trans-thiothixene. No trans-thiothixene was detectable and cis-thiothixene concentrations ranged from 0 to 22.5 ng/ml.
描述了一种高效液相色谱法，用于分离硫利达嗪（一种用作抗精神病药物的噻吨衍生物）的顺式和反式异构体。使用径向压缩模块（RCM-100）搭配硅胶柱和氰基丙基柱。在这些研究中使用固定波长紫外检测器（254nm）进行定量分析。由于美索达嗪的分离特性和可重复定量，将其用作内标。使用C18 Sep-Pak柱对生物样品进行净化处理。对接受硫利达嗪（甲硫达嗪）治疗的患者的血浆样本进行顺式和反式硫利达嗪检测。未检测到反式硫利达嗪，顺式硫利达嗪浓度范围为0至22.5ng/ml。
