Davis C M, Harrington C A
Analytical Neurochemistry Laboratory, University of Texas Mental Sciences Institute, Houston, TX 77030.
Ther Drug Monit. 1988;10(2):215-23. doi: 10.1097/00007691-198802000-00017.
A specific and sensitive assay procedure to measure thiothixene (Navane) in plasma has been developed and used to measure plasma concentrations in patients receiving thiothixene. The procedure involves in situ fluorescent detection after separation by high-performance thin-layer chromatography. Fluorescent detection permits a limit of detectability of approximately 0.1 ng/ml in plasma and the coefficient of variation is less than 6% at 2 ng/ml. Thirty samples may be processed through the entire procedure in less than 6-h period and up to 60 samples may be simultaneously spotted and chromatographed with a larger-capacity spotter and plate. Plasma levels (n = 62) drawn 10-12 h after dosage ranged from 0 to 42 ng/ml from dosages of 4-100 mg/day.
已开发出一种特异且灵敏的检测方法，用于测定血浆中的硫利达嗪（甲硫达嗪），并用于检测接受硫利达嗪治疗患者的血浆浓度。该方法包括在高效薄层色谱分离后进行原位荧光检测。荧光检测使血浆中的检测限约为0.1 ng/ml，在2 ng/ml时变异系数小于6%。在不到6小时的时间内可通过整个流程处理30个样本，使用更大容量的点样器和平板时，可同时点样和色谱分析多达60个样本。给药后10 - 12小时采集的血浆水平（n = 62），日剂量为4 - 100 mg时，范围为0至42 ng/ml。
