Newcomer C E, Anver M R, Simmons J L, Wilcke B W, Nace G W
Lab Anim Sci. 1982 Dec;32(6):680-6.
Chlamydial infection was suspected when widespread pyogranulomatous inflammation and large basophilic intracytoplasmic inclusion bodies were evident histopathologically in African clawed frogs (Xenopus laevis) dying of a spontaneous disease of high morbidity and mortality. Organism morphology was determined by electron microscopy of infected hepatic sinusoidal lining cells, and it was characteristic of the unique developmental cycle of a chlamydial agent. Isolation and speciation of the organism was achieved in a McCoy cell culture system. The infected cells were inoculated into disease-free frogs reproducing the disease.
当患有高发病率和死亡率的自发性疾病而死亡的非洲爪蟾（非洲爪蟾）在组织病理学上出现广泛的脓性肉芽肿性炎症和大的嗜碱性胞质内包涵体时，怀疑存在衣原体感染。通过对受感染的肝窦内衬细胞进行电子显微镜检查确定了病原体形态，其具有衣原体独特发育周期的特征。在 McCoy 细胞培养系统中实现了该病原体的分离和鉴定。将受感染的细胞接种到无病青蛙体内，引发了该疾病。
