Inhibitory effect of cytochalasin E on galactose absorption in the mouse in vitro.

The inhibitory effect of cytochalasin E on galactose absorption in everted sacs of mouse jejunum was studied. Cytochalasin B had highest potency on the inhibition of galactose absorption when it was added in the mucosal solution followed by cytochalasins E, A, C and D respectively. Cytochalasin E at a concentration as low as 2.5 micrograms/ml could inhibit galactose absorption by 17.1% within 60 min incubation and its maximum inhibition was obtained at a concentration of 10.0 micrograms/ml. At this concentration, it inhibited both Na+-dependent and independent galactose absorption. Our results in the present study and previously reported indicate that cytochalasin E inhibits the active intestinal absorption of galactose as well as glucose in the mouse.