Cytochalasin E: inhibition of intestinal glucose absorption in the mouse.

In situ glucose absorption in the mouse was significantly inhibited by cytochalasin E. Cytochalasin E (5 micrograms/ml) also inhibited glucose absorption up to 55.5% in mouse jejunum in vitro. During its inhibition transmural potential difference (PD) was increased from -7.4 to -0.4 mV, together with a decrease in glucose accumulation in the intestinal tissues. Furthermore, it was also found that cytochalasin E induced an alteration in Km value from 2.9 X 10(-3) to 4.0 X 10(-2) M and a constant Vmax value of 55.5 mumol/100 mg wet wt tissue/min. It is postulated that cytochalasin E is a possible competitive inhibitor of glucose at the receptor sites of carriers on the microvillar membrane of the intestinal absorptive cells.