Costa M, Cantoni O, de Mars M, Swartzendruber D E
Res Commun Chem Pathol Pharmacol. 1982 Dec;38(3):405-19.
In order of decreasing potency, CdCl2 greater than HgCl2 greater than CoCl2 greater than CuSO4 greater than NiCl2 greater than ZnCl2 and PbSO4 slowed cell growth at concentrations ranging from 1 microM to 60 microM. Flow cytometry analysis of cell cycle position indicated that cell growth was selectively blocked in S phase by these concentrations. Water insoluble metals such as As, Ni, crystalline Ni3S2, crystalline NiS, crystalline Ni3Se2 and NiO also resulted in an S phase blockage of cells at concentrations of 1 to 10 micrograms/ml. The crystalline nickel sulfide and selenide compounds as well as As metal were the most potent of these. At higher concentrations blockage of cells in mitosis was also evident with a number of the water insoluble metal compounds. The potency of the metal compounds in blocking cells in S phase was related to their chemical reactivity and their uptake into cells. The S phase specific blockage produced by the metals examined was consistent with their genotoxic or carcinogenic activity since such activity indicated a selective interaction with DNA metabolism.
按照效力递减顺序，氯化镉＞氯化汞＞氯化钴＞硫酸铜＞氯化镍＞氯化锌，硫酸铅在1微摩尔至60微摩尔浓度范围内会减缓细胞生长。细胞周期位置的流式细胞术分析表明，这些浓度会使细胞生长在S期被选择性阻断。不溶于水的金属，如砷、镍、结晶态的硫化镍（Ni3S2）、硫化镍（NiS）、硒化镍（Ni3Se2）和氧化镍，在1至10微克/毫升浓度时也会导致细胞在S期受阻。这些物质中，结晶态的硫化镍和硒化镍化合物以及金属砷效力最强。在较高浓度时，许多不溶于水的金属化合物也会使细胞在有丝分裂期受阻。金属化合物在阻断细胞S期方面的效力与其化学反应性及其进入细胞的情况有关。所检测的金属产生的S期特异性阻断与它们的基因毒性或致癌活性一致，因为这种活性表明它们与DNA代谢存在选择性相互作用。
