Zimmerman G L, Jen L W, Cerro J E, Farnsworth K L, Wescott R B
Am J Vet Res. 1982 Dec;43(12):2097-100.
An enzyme-linked immunosorbent assay (ELISA) was evaluated for diagnosis of Fasciola hepatica infections in sheep maintained under isolation and drylot conditions. Sheep were exposed to various numbers (100 to 500) of metacercariae, and blood was collected for assay at regular intervals thereafter. The ELISA values of controls were low and varied little. Although the ELISA detected antibodies to the flukes as early as postinoculation week (PIW) 2, ELISA values suggested as diagnostic (1.75 times control values) did not occur until PIW 6 to 8. Positive diagnosis of the infections by fecal examination for fluke eggs was not established until after PIW 10. Results also indicated that ELISA values were generally not correlated with magnitude of infection, concurrent nematode infections, or husbandry practices.
对酶联免疫吸附测定（ELISA）进行了评估，以诊断在隔离和干圈饲养条件下的绵羊肝片吸虫感染情况。绵羊接触了不同数量（100至500个）的囊蚴，此后定期采集血液进行检测。对照组的ELISA值较低且变化不大。尽管ELISA早在接种后第2周（PIW 2）就检测到了针对吸虫的抗体，但直到PIW 6至8才出现提示诊断的ELISA值（为对照值的1.75倍）。通过粪便检查吸虫卵对感染进行阳性诊断直到PIW 10之后才得以确定。结果还表明，ELISA值通常与感染程度、同时存在的线虫感染或饲养方式无关。
