Duménigo Ripoll B E, Finlay Villalvilla C M
Instituto de Medicina Tropical Pedro Kourí.
Rev Cubana Med Trop. 1998;50(1):82-4.
The diagnosis of ovine fascioliasis hepatica is presented by a sandwich ELISA with the use of monoclonal antibody ES78 previously reported for the diagnosis of bovine fascioliasis. The assay was applied to faeces of 40 sheep infected with Fasciola hepatica, 88 sheep with other parasitary infections tested by coproparasitology and 100 caprologically negative animals. Antigens of excretion-secretion of Fasciola hepatica were detected in 38 of the 40 sheep with fascioliasis and in none of the negative animals or with other parasitosis. The results show that ES78 sandwich ELISA, as in bovines, is fast, simple and useful for the diagnosis of the active infection caused by Fasciola hepatica among bovines.
使用先前报道用于诊断牛肝片吸虫病的单克隆抗体ES78的夹心ELISA法对绵羊肝片吸虫病进行诊断。该检测方法应用于40只感染肝片吸虫的绵羊粪便、88只经粪便寄生虫学检测患有其他寄生虫感染的绵羊以及100只经粪便学检测为阴性的动物。在40只患有肝片吸虫病的绵羊中，有38只检测到肝片吸虫的排泄-分泌抗原，而在阴性动物或患有其他寄生虫病的动物中均未检测到。结果表明，ES78夹心ELISA法与牛的情况一样，快速、简单且有助于诊断牛群中由肝片吸虫引起的活动性感染。
