Ozawa K, Kamiyama Y, Kimura K, Ukikusa M, Kono Y, Yamato T, Shimahara Y, Nakatani T, Asano M, Irie R, Kawashima S, Uchida K, Ohtoshi M, Aoyama H, Hirai F, Yasuda K, Tobe T
Artif Organs. 1982 Nov;6(4):447-52. doi: 10.1111/j.1525-1594.1982.tb04142.x.
The effect of liver support on the adenylate energy charge (ATP + 0.5ADP)/(ATP + ADP + AMP) of the remnant liver after major hepatic resection was studied in rabbits. The present study emphasized the principle of restoring the decreased energy charge level of the remnant liver after major hepatic resection by use of an ex vivo heterologous liver cross-hemodialysis with an interposed Cuprophan membrane. The energy charge level provides the cell with a very sensitive intracellular control mechanism. Regulatory enzymes from biosynthetic sequences exhibit very little activity at low levels of energy charge, and their activities increase sharply at high-energy charge levels. The energy charge level of the remnant liver maximally decreased from the control level of 0.860 to 0.767 at 24 hours after 70% hepatectomy. The energy charge level increased from 0.767 to 0.857 after two hours of cross-hemodialysis with an interposed Cuprophan membrane between the 24-hour, 70% hepatectomized rabbit and an ex vivo pig liver with high energy charge. The above results suggest that this ex vivo pig cross-hemodialysis may be effective for biosynthesis in the regenerative processes of the remnant liver.
在兔身上研究了肝支持对大肝切除术后残余肝脏腺苷酸能荷（ATP + 0.5ADP）/（ATP + ADP + AMP）的影响。本研究强调了通过使用插入铜仿膜的体外异种肝交叉血液透析来恢复大肝切除术后残余肝脏降低的能荷水平的原理。能荷水平为细胞提供了一种非常敏感的细胞内控制机制。来自生物合成序列的调节酶在低能荷水平时活性很低，而在高能荷水平时其活性急剧增加。70%肝切除术后24小时，残余肝脏的能荷水平从对照水平0.860最大降至0.767。在24小时、70%肝切除的兔与具有高能荷的体外猪肝之间插入铜仿膜进行两小时交叉血液透析后，能荷水平从0.767升至0.857。上述结果表明，这种体外猪交叉血液透析可能对残余肝脏再生过程中的生物合成有效。
