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用薄层色谱法和荧光分析法检测肉类中的群勃龙残留量(作者译)

[Detection of trenbolone residues in meat by thin layer chromatography and fluorimetry (author's transl)].

作者信息

Hohls F W, Stan H J

出版信息

Z Lebensm Unters Forsch. 1978 Oct 30;167(4):252-5. doi: 10.1007/BF01135598.

Abstract

An new method for the qualitative and quantitative determination of trenbolone residues in meat, liver and kidney is described. The analytical procedure consists of the following steps: comminution of the meat sample; homogenisation with tetrahydrofurane; liquid-liquid partition first between acetonitril and hexane then between sodium hydroxide solution and petroleum ether/benzene; chromatography on a silica gel column. The detection on the TLC-plate is performed with 366 nm ultra violet light. The quantitative determination is carried out by means of a remission spectral fluorimeter directly on the plate. The detection limit in meat extracts was 5 ppb.

摘要

描述了一种用于定性和定量测定肉类、肝脏和肾脏中群勃龙残留量的新方法。分析步骤包括以下几步:将肉类样品粉碎;用四氢呋喃均质化;先在乙腈和己烷之间进行液-液分配,然后在氢氧化钠溶液和石油醚/苯之间进行液-液分配;在硅胶柱上进行色谱分析。在硅胶薄层层析板上用366纳米紫外光进行检测。定量测定直接在板上用反射光谱荧光计进行。肉类提取物中的检测限为5 ppb。

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