Amaral D G, Foss J, Monjan A A, del Cerro M
Int J Neurosci. 1982 Sep;17(2):71-82. doi: 10.3109/00207458208985094.
Morphological changes in cerebellar neurons during the immune reaction to a viral infection were examined using Golgi methods. Wistar albino rats, infected at 4 days of age with lymphocytic choriomeningitis virus, were killed on postinfection days 4 to 10, and the cerebella were processed by rapid Golgi and Golgi-Cox methods. Some of the sections impregnated by the rapid Golgi method were subsequently processed for electron microscopy. Necrosis was evident in the lingula and portions of the posterior vermis by postinjection day 7. By the 9th day, degeneration was observed throughout the vermis. Purkinje cells appeared normal 4 days after infection, but by days 9 and 10, all the impregnated Purkinje cells had atrophic cell bodies; stunted, agglomerate dendrites; and dilated axons with collaterals that had many short processes. Some cells in the infected tissue did not resemble any normal cell type. These data indicate that the value of the Golgi method is a useful complement to conventional techniques for examining the neuropathological response to viral infections. In fact, it provides neuropathological information that is difficult or impossible to obtain with other procedures.
采用高尔基染色法，研究了病毒感染免疫反应过程中小脑神经元的形态学变化。4日龄的Wistar白化大鼠感染淋巴细胞性脉络丛脑膜炎病毒，在感染后第4至10天处死，取小脑用快速高尔基染色法和高尔基-考克斯染色法处理。部分经快速高尔基染色法浸染的切片随后进行电子显微镜检查。注射后第7天，在小脑舌叶和后蚓部部分区域可见明显坏死。到第9天，整个蚓部均观察到变性。感染后4天浦肯野细胞看起来正常，但到第9天和第10天，所有经浸染的浦肯野细胞均出现细胞体萎缩、树突短小且聚集、轴突及侧支扩张并有许多短突起。感染组织中的一些细胞与任何正常细胞类型均不相似。这些数据表明，高尔基染色法对于检查病毒感染的神经病理反应而言，是对传统技术的有益补充。事实上，它提供了用其他方法难以或无法获得的神经病理学信息。
