Harrison C J, Allen T D, Britch M, Harris R
J Cell Sci. 1982 Aug;56:409-22. doi: 10.1242/jcs.56.1.409.
Human metaphase chromosomes, prepared for light microscopy were examined by scanning electron microscopy. Use of an osmium impregnation technique eliminated the need for sputter-coating and allowed high-resolution visualization of uncoated specimens. Chromosomes were of three-dimensional cylindrical profile, with well-defined chromatids and centromeres. Prior to Giemsa-banding a smooth surface morphology was observed. Relaxation of chromosome integrity by Giemsa-banding pretreatment allowed resolution of several orders of chromosome structure not previously demonstrated by scanning electron microscopy. The observed organization of the chromatin fibres allowed parallels to be drawn with the radial loop model of chromosome construction as described by Marsden and Laemmli.
对为光学显微镜检查制备的人类中期染色体进行了扫描电子显微镜检查。使用锇浸渍技术无需进行溅射镀膜，并能对未镀膜的标本进行高分辨率观察。染色体呈三维圆柱形轮廓，染色单体和着丝粒清晰可辨。在吉姆萨染色之前，观察到表面形态光滑。吉姆萨染色预处理使染色体完整性松弛，从而能够分辨出扫描电子显微镜以前未显示的几个染色体结构层次。观察到的染色质纤维组织与马斯登和莱姆利描述的染色体构建的径向环模型有相似之处。
