Urdal P, Urdal K, Strømme J H
Scand J Clin Lab Invest. 1982 Dec;42(8):621-5.
The mass concentration of creatine kinase BB (CK-BB) isoenzyme was measured in serum of 365 healthy children and adults with a CK-BB specific radioimmunoassay. Eight separate age groups (newborns, 4 days, and 1, 8, 14, 25, 40 and 65 years) were examined and the reference intervals for these groups were estimated. The concentration of CK-BB generally decreased with increasing age, most of the changes taking place within the first year of life. Thus, the median value at age 1 year (9.2 micrograms/l) was 1/15th of that observed at birth, 1/2 of that observed at age 4 days, but four times higher than that observed in adults. A transient increase in CK-BB concentration was observed in boys 14 years old. At this age in contrast to at all other ages the values measured in boys were significantly (P less than 0.005) higher than the values measured in girls In 27 of the sera from cord blood we also examined the relationship between estimates of CK-BB mass and activity, the latter being estimated by immuno-inhibition technique. The two estimates were highly correlated (r = 0.98), 2 micrograms of enzyme mass roughly equivalent to 1 U of enzyme activity.
采用肌酸激酶BB（CK - BB）特异性放射免疫分析法，对365名健康儿童和成人血清中的CK - BB同工酶的质量浓度进行了测定。研究了八个不同年龄组（新生儿、4日龄、1岁、8岁、14岁、25岁、40岁和65岁），并估算了这些组别的参考区间。CK - BB的浓度通常随着年龄的增长而降低，大部分变化发生在生命的第一年。因此，1岁时的中位数（9.2微克/升）是出生时观察值的1/15，4日龄时观察值的1/2，但比成年人观察值高四倍。在14岁的男孩中观察到CK - BB浓度短暂升高。与所有其他年龄相比，在这个年龄，男孩测得的值显著高于女孩（P<0.005）。在27份脐血血清中，我们还研究了CK - BB质量估计值与活性之间的关系，后者通过免疫抑制技术进行估计。这两种估计值高度相关（r = 0.98），2微克酶质量大致相当于1个酶活性单位。
