Bergner U, Bergner H
Arch Tierernahr. 1982 Dec;32(12):841-52. doi: 10.1080/17450398209421033.
Test rats were labelled with 15N over a period of 7 days and killed on the 12th day. 220 min. before they were killed the diet was changed (wheat diet leads to whole egg diet) and they were given a 14C-leucine injection. The 15N-labelling of the digesta proved to be suitable for the determination of the digestibility of the feed proteins in the individual sections of the intestines. If the atom-% 15N-excess of the TCA-soluble fraction of the digesta is = 100%, the resulting digestibility of the protein fraction (TCA-precipitable fraction) corresponds to the 15N-dilution of the unlabelled feed protein. The following digestibility values were ascertained: (formula; see text) The calculation method suggested here cannot be applied to the large intestine because microbial activity influences the quotients. 14C-labelling was also insufficient for gathering statements on the digestibility of proteins.
给实验大鼠用15N标记7天，并在第12天处死。处死前220分钟改变饮食（从小麦饮食改为全蛋饮食），并给它们注射14C-亮氨酸。结果表明，消化物的15N标记适用于测定肠道各段饲料蛋白质的消化率。如果消化物的三氯乙酸可溶性部分的15N过量原子百分比=100%，则蛋白质部分（三氯乙酸可沉淀部分）的消化率对应于未标记饲料蛋白质的15N稀释。确定了以下消化率值：（公式；见正文）这里建议的计算方法不适用于大肠，因为微生物活性会影响商值。14C标记也不足以获取关于蛋白质消化率的信息。
