Valerius N H
Acta Pathol Microbiol Scand B. 1978 Jun;86B(3):149-54.
The effect of colchicine on human neutrophil granulocyte chemotaxis, chemokinesis and spontaneous motility was examined, using a modified reversible Boyden chamber. Colchicine was shown to inhibit the attraction of neutrophils to casein and to a bacterial chemotactic factor at concentrations as low as 10(-7) M. Experiments in which the absolute concentrations and the concentration gradients of the chemotactic agent were varied, revealed that colchicine inhibited chemokinesis rather than chemotaxis. The spontaneous motility measured in the absence of chemotactic agents was not inhibited by colchicine. Pre-incubation of the cells with a bacterial chematactic factor did not change the sensitivity of the cells to colchicine. It is concluded that the integrity of microtubule function is not necessary for the ability of the cells to discern a concentration gradient or to react to this with directional locomotion. Thus the inhibitory effect of colchicine on neutrophil granulocyte chemokinesis may not depend on its inhibition of microtubule function. It is suggested that colchicine may block the still unidentified membrane mechanism involved in the translation of the recognition signal into an appropriate locomotory cell response.
使用改良的可逆博伊登小室研究了秋水仙碱对人中性粒细胞趋化性、化学增活现象和自发运动的影响。结果表明，秋水仙碱在低至10^(-7) M的浓度下就能抑制中性粒细胞对酪蛋白和细菌趋化因子的吸引。改变趋化剂绝对浓度和浓度梯度的实验表明，秋水仙碱抑制的是化学增活现象而非趋化性。在无趋化剂情况下测得的自发运动不受秋水仙碱抑制。用细菌趋化因子对细胞进行预孵育不会改变细胞对秋水仙碱的敏感性。结论是，微管功能的完整性对于细胞识别浓度梯度或对此作出定向运动反应的能力并非必需。因此，秋水仙碱对中性粒细胞化学增活现象的抑制作用可能不依赖于其对微管功能的抑制。有人提出，秋水仙碱可能会阻断将识别信号转化为适当的运动细胞反应所涉及的尚未明确的膜机制。
