Aonuma S, Okabe M, Kishi Y, Kawaguchi M, Yamada H
J Pharmacobiodyn. 1982 Dec;5(12):980-7. doi: 10.1248/bpb1978.5.980.
The role of serum albumin in in vitro fertilization of mouse ova was studied relating to the fertilization inhibitory activity of Zn2+. The time required for mouse sperm capacitation was dependent on the concentration of serum albumin which was added to the medium. The majority of Zn2+ added to the medium was shown to be bound to serum albumin (95% out of 200 microM) by means of equilibrium dialysis. Furthermore, it was suggested that the serum albumin was necessary for the initiation of the sperm capacitation process by the periodic observation of sperm penetration through zona pellucida.
研究了血清白蛋白在小鼠卵子体外受精中与锌离子受精抑制活性相关的作用。小鼠精子获能所需时间取决于添加到培养基中的血清白蛋白浓度。通过平衡透析表明，添加到培养基中的大部分锌离子（200微摩尔中的95%）与血清白蛋白结合。此外，通过定期观察精子穿透透明带发现，血清白蛋白对于启动精子获能过程是必要的。
