锌离子对小鼠卵子体外受精的影响。
The effect of zinc ions on fertilization of mouse ova in vitro.
作者信息
Aonuma S, Okabe M, Kawaguchi M
出版信息
J Reprod Fertil. 1978 May;53(1):179-83. doi: 10.1530/jrf.0.0530179.
PMID:580440
Abstract
The fertilization rate of mouse gametes in a Krebs-Ringer bicarbonate medium was 60%. The rate fell to 0% when 250 micron-ZnCl2 was added, but MgCl2, CaCl2, MnCl2, FeCl2, and CuCl2 (each at 250 micron) had no effect. The inhibitory effect of Zn2+ was not evident if the spermatozoa or eggs were preincubated with Zn2+ or if previously capacitated spermatozoa were used. The Zn2+ is thought to act by interference with capacitation.
摘要
在 Krebs-Ringer 碳酸氢盐培养基中，小鼠配子的受精率为 60%。添加 250 微米的 ZnCl2 后，受精率降至 0%，但添加 250 微米的 MgCl2、CaCl2、MnCl2、FeCl2 和 CuCl2 则无影响。如果精子或卵子预先与 Zn2+ 孵育，或者使用先前已获能的精子，则 Zn2+ 的抑制作用不明显。据认为，Zn2+ 通过干扰获能发挥作用。
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