Chaicumpa W, Peungjesda U, Martinez B, Atthasishtha N
Southeast Asian J Trop Med Public Health. 1982 Dec;13(4):637-45.
Soluble haemagglutinin was isolated from a late culture supernatant of classical Vibrio cholerae grown in casein-peptone broth with aeration by saturated ammonium sulphate precipitation in the cold, followed by gel filtration, electrophoresis, ether extraction and electrophoresis respectively. The antigen was found to consist of three protein fractions with different electrophoretic mobilities. Antibodies to these fractions afforded low but significant protection against oral challenges with homologous classical V. cholerae in the infant mouse cholera model.
通过在低温下用饱和硫酸铵沉淀，随后分别进行凝胶过滤、电泳、乙醚萃取和电泳，从在酪蛋白-蛋白胨肉汤中通气培养的古典霍乱弧菌晚期培养上清液中分离出可溶性血凝素。发现该抗原由具有不同电泳迁移率的三个蛋白质组分组成。针对这些组分的抗体在幼鼠霍乱模型中对同源古典霍乱弧菌的口服攻击提供了低但显著的保护作用。
