Temperature-dependent variation in the synthesis of the receptor for Yersinia enterocolitica bacteriophage XI.

Growth temperature affected the synthesis of the receptor for Yersinia enterocolitica bacteriophage XI. The cell wall preparation from the cells grown at 25 degrees C inactivated the bacteriophage, whereas cell walls grown at 37 degrees C did not. The alkaline extraction was performed with 0.03 M sodium hydroxide. The alkaline extract from the 25 degrees C-grown cells required for 50% phage inactivation amounted to 10 micrograms, whereas the compound from the 37 degrees C-grown cells did not inactivate the bacteriophage. It was indicated by diethylaminoethyl cellulose column and Sephadex G-200 column chromatographic analyses that the receptor material was extracted as a sugar-protein complex by the alkaline treatment.