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所选噬菌体对小肠结肠炎耶尔森菌菌株的活性。

The activity of chosen bacteriophages on Yersinia enterocolitica strains.

作者信息

Kot B, Bukowski K, Jakubczak A, Kaczorek I

机构信息

Department of Microbiology, University of Podlasie, 12 Prusa Street, 08-110 Siedlce, Poland.

出版信息

Pol J Vet Sci. 2002;5(1):47-50.

Abstract

The aim of the present study was to evaluate the lytic activity of three bacteriophages on Yersinia enterocolitica strains isolated from humans and pigs. The Y. enterocolitica strains tested belonged to 0:3, 0:9 and 0:2 serogroups. The ZD5 phage was obtained from a water sample, but remaining phages were obtained from the lysogenic Y. frederiksenii 7291 and Y. enterocolitica 8684 strains. All the Y. enterocolitica strains tested which belonged to 0:9 serogroup did not show any susceptibility to the bacteriophages used. The bacteriophages tested showed different lytic activity on the Y. enterocolitica 0:3 strains investigated. The phage susceptibility of Y. enterocolitica 0:3 strains revealed 9 different phage patterns. ZD5 phage showed the highest lytic activity, because it produced confluent lysis of the most Y. enterocolitica 0:3 strains tested. The Y. enterocolitica 0:2 strains isolated from pigs showed the similar phage susceptibility. The Y. kristensenii and Y. pseudotuberculosis strains tested were not sensitive to the bacteriophages used.

摘要

本研究的目的是评估三种噬菌体对从人和猪中分离出的小肠结肠炎耶尔森菌菌株的裂解活性。所测试的小肠结肠炎耶尔森菌菌株属于0:3、0:9和0:2血清群。ZD5噬菌体是从水样中获得的,但其余噬菌体是从溶原性弗氏耶尔森菌7291和小肠结肠炎耶尔森菌8684菌株中获得的。所有测试的属于0:9血清群的小肠结肠炎耶尔森菌菌株对所用噬菌体均无敏感性。所测试的噬菌体对所研究的小肠结肠炎耶尔森菌0:3菌株表现出不同的裂解活性。小肠结肠炎耶尔森菌0:3菌株的噬菌体敏感性显示出9种不同的噬菌斑模式。ZD5噬菌体表现出最高的裂解活性,因为它对大多数测试的小肠结肠炎耶尔森菌0:3菌株产生了融合裂解。从猪中分离出的小肠结肠炎耶尔森菌0:2菌株表现出相似的噬菌体敏感性。所测试的克氏耶尔森菌和假结核耶尔森菌菌株对所用噬菌体不敏感。

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